Yes, but the freezer will shrink the soap a bit so you do not want to put it in the freezer between layers. EX) If you have poured a white layer & place it in the freezer, it will shrink & pull slightly away from the sides, so if you go back & pour a second layer of say, red, that will ooze down between the side of your mold & the 1st later & you will have a mess.